Phd thesis cell culture

Bennett, Malcolm J. Barrett, A. Barstow, David A.

Cancer cells in 3D cell culture (breast adenocarcinoma)

Bright, John The physiological activity of attached bacteria. Bains, William The structure of cloned histone genes of Xenopus borealis. Barlow, Denise P. Baker, Phillip Nigel A study on the control of interferon production in lymphoblastoid cells. Blair, G. Eric Protein synthesis in chloroplasts. Brown, Richard Henry A study of the cell envelope of Halobacterium salinarium.

Carbonero, Franck Distributions of model microorganisms along an estuarine gradient. Carson, Connor Vaccine trials against canine leishmaniasis. Chan, Jacqueline Bacteriophages of marine Roseobacter.

Institute of Microbiology and Biotechnology

Cowan, Richard H. Canning, Peter The prolyl oligopeptidase family enzymes: structural basis of inhibition. Cavilla, Jennifer Louise The effects of factors influencing human oocyte maturation upon fertilization and preimplantation embryo development. Chen, Jing Expression of corticotrophin-releasing hormone receptor subtypes in human myometrium and cloning of the promoter region for the CRH receptors type 2. Callaghan, Anastasia J. Cooper, Ben The transmission dynamics of methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci in hospital wards. Chant, Ian David Heat shock protein expression and apoptosis in myeloid leukaemias.

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Cole, James Francis Thompson The surface dynamics of the northern Benguela upwelling system and its relationship to patterns of clupeoid production. Clark, Darren Alan The study of acidophilic, moderately thermophilic iron-oxidizing bacteria.

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Chadd, Helen E. Chaddock, John Andrew Structure-function relationships of ricin A-chain. Cox, Simon Peter Iron oxidation and mineral oxidation by moderately thermophilic bacteria. Caravokyri, Calliope Characterization of temperature-sensitive mutants of human respiratory syncytial RS virus. Chambers, Stephen Alexander The production, purification and catalytic utility of lignin peroxidase from "sporotrichum pulverulentum".

Cardy, Donald Leonard Nicholas The molecular biology of ammonia assimilation in the obligate methanotroph Methylococcus capsulatus strain Bath. Cardy, Susan Mary Lactobacillus leichmannii as a probe for the quantitation of vitamin B Clements, Gary James Studies on the entry of ricin subunits into cells. Crickmore, Neil Analysis of a cell division gene cluster in "Escherichia coli".

Carter, Andrew T. Conder, Michael John Origin of glyoxysomal membrane proteins in castor bean endosperm. Cross, Gareth S. Clarke, Ian Nicholas Genomic variation in rotaviruses. Cutler, Daniel Franklin The secretion of chick proteins from Xenopus oocytes : an investigation into non-parallel secretion. Di Maiuta, Nicola Characterisation of mixed microbial populations in white mineral dispersions. Durrant, Oliver Structural investigation of the Arabidopsis thaliana circadian clock. Davies, Marc Tudor Subcellular location and protein interactions of the infectious bronchitis virus gene 3 and 5 accessory proteins.

Dumont, Marc G. Dee, Chris T. Dimmock, John Functional interactions of the adenovirus serotype 5E4orf3 protein. Delaney, Michael Paul Immunosuppressive drug interactions and resistance in mononuclear cells from renal transplant patients. MD thesis, University of Warwick. Drummond, James A. Dix, Ian The expression and function of the adenovirus type 5 E4 region. Davidson, Sylvia The development of genetic techniques for the obligate methanotroph, Methylococcus Capsulatus Bath.

Derbyshire, Paul Plasmid maintenance in Escherichia coli K Davey, John The accumulation of the influenza virus nucleoprotein in the nuclei of 'xenopus' oocytes. Dow, Crawford S. Evans, Charlotte Marie The impact of respiratory disease on production in the pig industry in Great Britain. Edworthy, Nicole Lynn Development and use of avian pneumovirus reverse genetics systems. Essa, Sahar Sultan A study on the cell mediated immunity of human cytomegalovirus infection in kidney transplant recipients. Elderfield, Peter D. Franklin, Mark P. Fuller, Nicholas J.

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Flaxman, Christine Susan A study of the regulation of undecylprodigiosin biosynthesis in Streptomyces coelicolor A3 2. Fox, Sian L. Graham, James Fionnlagh An investigation into the subcellular localisation of co-factors that stimulate prion protein conversion.

Gao, Anna Population biology of Propionibacterium acnes and Pseudomonas aeruginosa in ophthalmic infections and the development of novel diagnostic tools. Gargum, Ramadan Suliman Isolation and characterisation of thyroid hormone-responsive genes of amphibian tail. Ghosh, Monimoy The role of apoptosis and expression of bc12 and CD40 in squamous cell carcinoma of the lung. Gibson, F. Elizabeth The development of genetic systems for iron-oxidizing, acidophilic moderate thermophiles.

Gibbs, Thomas W. Gorham, Hazel Claire Characterisation of a novel bacteriophage, 0BHG1 : and its interactions with its host "Rhodopseudomonas blastica". Hart, Philip John The cytosolic fate of ricin A chain in target cells. Harris, Alasdair Analysis of recovery patterns of Indian Ocean coral reefs through examination of scleractinian communities and populations.

Hilton, Gillian R. Holland, Richard J.

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Huckstepp, Robert T. Hills, Claire E. Hardman-Mountford, Nicholas John Environmental variability in the Gulf of Guinea large marine ecosystem: physical features, forcing and fisheries. Heath, Richard Rhead The role of the cell-mediated immune response to rotavirus infection. Hendry, Ruth Juliet Spatial modelling in plant ecology. Herron, Paul Robert Interactions between actinophage and streptomycetes in soil.

Hay, Steven C.

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Howard, Katharine Mary Carbon metabolism and growth of marine unicellular cyanobacteria belonging to the genus "Synechococcus". Holness, Claire Louise Lesley Isolation and characterisation of mutants of cowpea mosaic virus. Hartree, David E. Hunt, Nicholas Molecular analysis of the Friend virus complex. Horrell, Ann Translational control of the heat shock response in Xenopus oocytes.

Heine, Christopher Retroviruses and insect cells. Hicks, Nigel John Studies on the effects of interferon on the phenotype or mouse fibroblasts that have been transformed by a murine sarcoma virus.

Irwin, Stephen D. Jia, Ying An investigation into the adsorption of cyanophages to their cyanobacterial hosts. Jackson, Julia Emma The development of methods for the selective capture and characterisation of fragrances and flavours from nature. Jakubovics, Nicholas S. Jolliffe, Alistair S. The option to use a cell type that is more biologically similar to the human in vivo situation, but can be maintained like cell lines, is a clear benefit to in in vitro assays. It was concluded that macrophages differentiated from induced pluripotent stem cells would be a suitable alternative to currently used cell types.

Copy to clipboard. Additional Information. Download Accepted Version Download. Google Scholar. Report this Record Edit item admin only View this record in elements. Co-culture in a 3D micromass system stimulated the osteogenic differentiation of progenitor cells, while in monolayer this was not seen. While the stimulation of MSCs with inflammatory and chemotactic factors successfully regulated the cell gene expression and secretion profile, no effect of the secrotome on the osteogenic differentiation of unstimulated cells in monolayer was demonstrated.

Altogether, these results indicated the importance of cell-to-matrix interconnectivity. Therefore, the last part of this thesis focused on the assessment of osteogenic differentiation in 2D and 3D cell culture models, which are physiologically relevant. The progression in osteogenesis depends on the applied 3D culture model. While in both, micromass and type I collagen-hydroxyapatite gel, the differentiation is enhanced compared to monolayer, the regulation of this process is triggered in a different manner in these 3D culture models.

Together these findings demonstrate how diverse outcomes can be obtained by the application of different models in in vitro research.